Techniques for microbiological cultures

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Sulhazan Staff asked 11 months ago

What are the Techniques for microbiological cultures

1 Answers
Sulhazan Staff answered 11 months ago

Microbiological culture can be easily isolated or proliferate using inoculation techniques.
Inoculation enables to isolate pure cultures from a mixture of different microorganisms or prolonged maintain bacteria on solid medium. An indispensable tool in any microbiology laboratory is the inoculating loop. The loop is a piece of wire that is looped at one end. By heating up the loop in an open flame, the loop can be sterilized before and after working with bacteria . Thus, contamination of the bacterial sample is minimized. The inoculating loop is part of what is known as aseptic (or sterile) technique. Another staple piece of equipment is called a petri plate. A petri plate is a sterile plastic dish with a lid that is used as a receptacle for solid growth media.
Cultures surface:

  • Streak plate, performed by transferring the microbial biomass from the culture liquid or solid substrate sterile solid support, drawing the eye loop shape in the form of “zigzag”. Inoculation is performed from a sector in order to isolate a pure culture of bacteria.
  • Mold culture is performed by transferring spores or piece of mycelium on the substrate surface in one centrally located place on solid medium or in three places, equally-spaced from one another.
  • The lawn (carpet) culture performed by transferring 0.1 ml of cell suspension on the surface of the solid substrate and uniform distribution over the entire surface using a sterile cotton swab or glass spreader sterilized by flaming in alcohol.
    The pour plates method – 1 ml of cell suspension is introduced into the center of a dry and sterile Petri dish, and then on the same plate agar medium is poured at a temperature of approx. 45 °C and gently stirred to evenly distribute the agar and microbiological material.